Indian Journal of Radiology and Imaging Indian Journal of Radiology and Imaging

NEURORADIOLOGY
Year
: 2011  |  Volume : 21  |  Issue : 3  |  Page : 202--208

Novel positron emission tomography radiotracers in brain tumor imaging


Maria Mathew D'Souza, Rajnish Sharma, Madhavi Tripathi, Puja Panwar, Abhinav Jaimini, Anupam Mondal 
 Division of PET Imaging, Molecular Imaging and Research Centre, Institute of Nuclear Medicine and Allied Sciences, Delhi, India

Correspondence Address:
Maria Mathew D«SQ»Souza
Division of Positron Emission Tomography Imaging, Molecular Imaging and Research Centre, Institute of Nuclear Medicine and Allied Sciences, Delhi - 110 054
India

Abstract

Although [18F] 2-fluoro-2-deoxy-D-glucose (FDG) is the most widely used radiopharmaceutical the world over, it is not the ideal tracer for brain imaging, owing to its high physiological cortical uptake and lack of specificity. This has paved the way for the introduction of several novel radiotracers, each with their own inherent strengths and limitations. We present the insights gained from the use of these radiotracers at our institution.



How to cite this article:
D'Souza MM, Sharma R, Tripathi M, Panwar P, Jaimini A, Mondal A. Novel positron emission tomography radiotracers in brain tumor imaging.Indian J Radiol Imaging 2011;21:202-208


How to cite this URL:
D'Souza MM, Sharma R, Tripathi M, Panwar P, Jaimini A, Mondal A. Novel positron emission tomography radiotracers in brain tumor imaging. Indian J Radiol Imaging [serial online] 2011 [cited 2019 Oct 15 ];21:202-208
Available from: http://www.ijri.org/text.asp?2011/21/3/202/85369


Full Text

 Introduction



Positron emission tomography/computerized tomography (PET/CT) has gained widespread acceptance in tumor evaluation owing to its ability to detect tumors and define their extent, provide information that aids planning of radiotherapy and operative interventions, show response to treatment, and detect tumor recurrence. [18F] 2-fluoro-2-deoxy-D-glucose (FDG) has been aptly called the molecule of the millennium because of the impact it has had in the field of oncological imaging. In fact, the first oncological application of PET was in the assessment of brain tumors. [1] FDG PET/CT has been utilized primarily in brain tumors for grading and characterization, prognostication, guiding the selection of the site for biopsy, and evaluation of therapeutic response with modest accuracy. However, it has its limitations, primarily the lack of specificity [2] and the high physiological cortical uptake. This has led to the development and utilization of newer non-FDG radiopharmaceuticals. We present the insights gained with the use of these non-FDG radiopharmaceuticals in brain tumor evaluation at our institution.

 Discussion



At present FDG is the most widely used radiopharmaceutical the world over. This is due its favorable half-life of 110 minutes (which does not necessitate an on-site cyclotron), high uptake in tumor tissue, and relatively low positron range. The basis for high FDG uptake in tumor tissue is the high metabolic rate and therefore increased glucose utilization in tumor cells. However, FDG may not be an ideal imaging agent for brain tumors as there is a high physiological glucose uptake in normal brain parenchyma, glucose being an obligatory energy substrate for brain. This leads to intense radiotracer uptake in normal brain tissue, and, as a result, low grade tumors, small tumors, and tumors with early recurrence may go undetected. Moreover, FDG uptake is relatively nonspecific and is seen to occur in inflammatory and granulomatous tissues also.

A variety of non-FDG PET radiotracers with oncological applications have been developed. These include radiotracers for amino acid synthesis, Deoxyribonucleic acid (DNA) synthesis, lipid synthesis, hypoxia, angiogenesis, and peptide receptors.

Radiotracers for amino acid synthesis

11 C methionine

Protein synthesis is a fundamental prerequisite for tumor growth. There is an upregulation of protein metabolism and cell-mediated transport of amino acids in tumors. Radiotracers that can measure the increase in protein synthesis will have increased sensitivity and specificity as compared to FDG. Several such radiopharmaceuticals have been developed. Of these, 11 C-labelled methionine has been the most widely studied. Unlike with FDG, the normal brain parenchyma exhibits overall reduced uptake of 11 C methionine. The pituitary gland shows increased uptake [Figure 1]. It has thus proven superior to FDG in the evaluation of brain tumors as its reduced uptake in healthy brain tissue results in enhanced contrast between the brain tumor and the surrounding normal parenchyma. [3] {Figure 1}

Despite its obvious advantages, 11 C methionine has certain limitations. The short half-life of twenty minutes necessitates an on-site cyclotron. Further, it takes some time for the radiotracer 11 C to bind to various physiological substrates, further reducing the amount of radioisotope available in the final radiopharmaceutical by the time it is ready for injection. However, it has been found to be more efficacious than FDG PET/CT both for the primary detection of tumor and for delineation of its extent [Figure 2]. Additionally, it can differentiate tumorous from nontumorous lesions with a high degree of sensitivity and specificity. [4] A semiquantitative estimate of the level of radiopharmaceutical uptake in a particular tissue can be made by calculating the standardized uptake value (SUV). The ratio of radiotracer uptake in the pathological region to that in the contralateral uninvolved cortex can then be calculated. A threshold of 1.48 of relative 11 C methionine uptake between tumor and normal tissue has been found to yield a high sensitivity of 83% and a specificity of 92%. [5] {Figure 2}

This is of particular relevance in the Indian setting, where the incidence of intracranial tuberculomas and other inflammatory granulomas is high. These lesions often mimic tumors clinically as well as on imaging studies. 11 C methionine has demonstrated good results in distinguishing tuberculomas from neoplasia [Figure 3] and [Figure 4].{Figure 3}{Figure 4}

Owing to its selective uptake in brain tumors, 11 C methionine PET/CT has also been found to be a useful modality for assessing the size and margins of gliomas that may not enhance on contrast-enhanced Magnetic resonance imaging (MRI) imaging. [6] It also plays an important role as a marker for cell proliferation and angiogenesis [5] and can be used to evaluate tumor grade in gliomas, thus allowing better tumor prognosis. [6] It plays an important role when planning the radiotherapy field and for guiding selection of the site of biopsy. 11 C methionine is useful for discriminating between tumor recurrence and radiation necrosis [Figure 5] and [Figure 6], a situation where CT scan, MRI, and FDG PET are often inconclusive. [7] However, there have been a few instances of 11 C methionine uptake in nonmalignant conditions as well, [8] such as brain abscesses and conditions wherein the blood-brain barrier is disrupted as in intracranial hematomas. The increased radiotracer accumulation noted in some of these cases may be a reflection of overall increased cell metabolism, although the exact mechanism is unclear [Figure 7]. {Figure 5}{Figure 6}{Figure 7}

Other amino acid tracers

Malignant transformation increases the use of aminoacids for energy, protein synthesis and cell division. Owing to the short half life of 20 minutes that 11 C methionine has, its use has been limited to institutions with an on-site cyclotron. Aminoacids labeled with a radiotracer having a longer half-life would be preferred in the routine clinical setting. Of these, 3,4-dihydroxy-6- 18 F-fluoro-L-phenylalanine (FDOPA) and O-(2-[18F]fluoroethyl)-l-tyrosine (FET) have been widely studied. Their uptake is mainly related to carrier mediated active transport, which is upregulated in tumor cells. [9] These amino acids are retained in tumor cells, which exhibit higher metabolic activities than most normal cells.

The superiority of FDOPA PET over FDG PET for imaging low-grade tumors and evaluating recurrent brain tumors has been demonstrated in past studies [10] [Figure 8] and [Figure 9]. Low-grade tumors are frequently FDG negative. However, as both high- and low-grade tumors show increased avidity for this radiotracer, it may not be useful for assessing tumor grade. One important advantage however is that tracer uptake is independent of blood-brain barrier breakdown, thus facilitating radiotracer uptake independent of tumor enhancement on contrast MRI studies. [11]{Figure 8}{Figure 9}

FET PET when combined with MRI significantly improves the identification of cellular glioma tissue. [12] In addition, high- and low-grade brain tumors can be differentiated on the basis of the different uptake kinetics of FET. It has also proven useful in differentiating malignant from benign lesions of the brain. [13]

Radiotracers for Deoxyribonucleic acid synthesis

DNA synthesis is an important prerequisite for cellular proliferation. The most widely used radiotracer for assessing DNA synthesis is 3-deoxy-3-[18F] flurothymidine (FLT). This compound gets phosphorylated by thymidine kinase, which shows markedly increased activity in proliferating tumors, and thereafter gets trapped within the cell. The imaging of cellular proliferation has a potential advantage over glucose imaging because FLT is specific to tumors, while high levels of energy metabolism are also seen with other processes including inflammation. [14] Thus, unlike FDG it does not show uptake into inflammatory cells and has been widely used to distinguish benign from malignant pulmonary lesions. Although this differentiation may not always be feasible, the possibility of using FLT for monitoring treatment with cytostatic anticancer drugs needs to be explored. [15] Like 11 C methionine, the background uptake in normal brain parenchyma is low, thus enhancing tumor detection [Figure 10]. {Figure 10}

Radiotracers for lipid synthesis

11 C- and 18 F-labeled choline have been used in brain tumors. The basis for this is the increased transport and phophorylation of choline in tumor cells, where it gets incorporated into the phospholipids of the cell membrane. It has been found useful for the differentiation of low-grade from high-grade gliomas, but not for distinguishing low-grade gliomas from nonmalignant lesions. [16] In a comparative study of FDG, 11 C methionine, and 11 C choline in 95 gliomas, 11 C methionine was found to be the most superior among the three. [17]

Radiotracers for hypoxia

Adequate tumor vascularization is an important precondition to tumor growth. Inadequate vascularization would culminate in tumor hypoxia and eventual necrosis. Hypoxic tissue is inherently more resistant to chemotherapy or radiotherapy and this is often responsible for failure of chemo-radiotherapy and an overall poor response. Several in vivo PET tracers have been developed to assess tumor hypoxia, e.g., [18] F fluoromisonidazole (FMISO) and 64/60Cu(II)-diacetylbis (N-4-methylthiosemicarbazone) (64/60Cu-ATSM), which have a propensity to accumulate in hypoxic rather than normoxic cells. [18] The most extensively used radiotracer for hypoxia is FMISO [Figure 11] and [Figure 12]. [19] Inclusion of FMISO imaging data provides information that is complementary to FDG PET data by correlating metabolic activity to tumor hypoxia. In a comparative study on glioblastomas, the biological aggressiveness assessed by serial MRI was seen to be linked with the hypoxic tumor burden assessed on FMISO_PET. [20] This aids the selection of alternative treatments and monitoring of their therapeutic efficacy.{Figure 11}{Figure 12}

Other radiotracers

The next generation of PET tracers includes those that will bind to specific cancer-related receptors or antigens. [2] These agents would offer tremendous opportunities for selective imaging, thus increasing the sensitivity and specificity for a particular tumor. There would also be enormous potential for directing molecular therapies targeted at these neoplasms. The development of targeted radiolabeled drugs to explore the efficacy of anticancer regimens holds promise for the future.

 Conclusion



A multitude of PET radiotracers has emerged for the assessment of brain tumors, each with its inherent strengths and weakness. There is a pressing need to undertake comparative trials of the various tracers so that we can select the best tracer for any given clinical situation.

 Acknowledgements



The authors gratefully acknowledge the contributions of Dr. RP Tripathi, Dr. Raunak and Dr. Anil Mishra, Institute of Nuclear Medicine and Allied Sciences.

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