| Abstract|| |
With the advent of newer imaging modalities retained surgical items are now easily diagnosed by their characteristic imaging appearances. A combination of complementary imaging modalities helps to arrive at the diagnosis of this relatively rare complication. Factors contributing to their imaging features include the timing of diagnosis and imaging, presence of secondary infection, communication of the retained item with hollow viscus or external skin wound, and type of imaging modality used. A high index of suspicion is necessary for diagnosis before labeling it as a retained surgical item. In parallel with recent advances in surgery, it is essential that there is increasing awareness among radiologists regarding the newer types of retained surgical items.
Keywords: Calcified; computed tomography; differential diagnosis; gossypiboma; imaging; magnetic resonance imaging; PET CT; retained surgical items; USG
|How to cite this article:|
Santhosh Kumar G V, Ramani S, Mahajan A, Jain N, Sequeira R, Thakur M. Imaging of retained surgical items: A pictorial review including new innovations. Indian J Radiol Imaging 2017;27:354-61
|How to cite this URL:|
Santhosh Kumar G V, Ramani S, Mahajan A, Jain N, Sequeira R, Thakur M. Imaging of retained surgical items: A pictorial review including new innovations. Indian J Radiol Imaging [serial online] 2017 [cited 2020 May 29];27:354-61. Available from: http://www.ijri.org/text.asp?2017/27/3/354/215576
| Introduction|| |
The term gossypiboma is derived from the Latin word “gossypium” meaning cotton wool, the suffix -oma meaning mass. Some authors also suggest that the latter part comes from the Swahili word “boma” which means concealment; the reference to the source is not clarified. Other terms used are “textiloma,” “gauzoma,” “muslinoma.” A suitable example of a gossypiboma would be a mass-like reaction to surgical sponges that are accidentally left inside the patient during surgery. With the introduction of a newer and broader term “retained surgical items” (RSI), additional materials such as needles, broken instruments, irrigation sets, and rubber materials have been included in the conventional list.
ICD-10 (International Statistical Classification of Diseases and Related Health Problems) classifies retained foreign bodies/objects and related complications in blocks from T81.5 to T81.6.
The incidence of RSI is difficult to estimate as patients may be asymptomatic as well as due to under-reporting of cases. The reported incidence varies between 1 per 1000 and 1 per 3000 procedures. The wide variation in the incidence depends on the type of procedure, experience of the surgeon and operation theatre personnel, hospital policies, frequency of reporting of adverse incidences, among other factors. Even though it is impossible to eliminate the factor of human error completely, the incidence of this rare but serious complication can be significantly reduced by following the recommended perioperative guidelines and checklists. There are very few reported publications on gossypiboma/RSI due to medicolegal implications. Awareness among surgeons and radiologists can lead to early diagnosis and intervention, thus preventing further complications. In this article, different imaging appearances of gossypiboma/RSI are presented including examples of newer RSI.
| Pathophysiology|| |
Any foreign body elicits a reaction in the human body. If the foreign body harbors microorganisms, it can introduce infection. The foreign body can also get secondarily infected. The type of reaction elicited depends on antigenicity (depending on the content of the retained item) and the presence of secondary infection. Two reactions classically described with RSI are exudative and fibrinous. The schematic flow chart of natural history of gossypibomas is showing in [Figure 1]., The exudative inflammatory reaction presents early, with or without secondary infection. Once the exudative reaction is initiated, it progresses with cytokine and white blood cell interactions, acute and chronic inflammation, and end-stage fibrous capsule development. The second reaction is fibrotic/fibrinous, which is characterized by a foreign body granuloma, adhesions, and encapsulation. Between the two reactions, the exudative reaction presents early with symptoms, facilitating early detection and surgical removal.
Natural history of RSI, depends on either of the above reactions, with some cases presenting in the early postoperative period. Some patients may be asymptomatic for a long time. In a few cases, retained sponges may erode the walls of viscera and migrate into the intestinal lumen due to pressure necrosis and granuloma formation and may be further propelled by peristalsis. Several reported cases of spontaneous transmural migration of gossypibomas and expulsion by defecations have been published.
| Clinical Presentations|| |
Surgical items can be accidentally left after any type of surgical procedure, with the most common site being abdomen and pelvis.,,, Other sites include skull, neck, thorax, breast, axilla, vagina, and paraspinal region. Clinical presentation depends on the site of gossypiboma/RSI and the time of presentation. Clinical presentation of abdominal gossypiboma/RSI varies from asymptomatic to emergency presentation usually in the form of abdominal pain. In acute manifestations, the patient usually presents with nonspecific abdominal pain, fever, vague abdominal lump, nausea, vomiting, abdominal wound discharge, or sepsis.
Patients can also present with complications arising from gossypiboma such as intestinal obstruction, perforation, gastrointestinal hemorrhage, peritonitis, or septic shock.
Subacute to chronic presentation includes vague chronic pain, anorexia, weight loss, or an abdominal mass, which can be misdiagnosed as a malignant tumor. Gossypibomas of other sites such as intrathoracic cavity, paraspinal area, cranium, breast, and neck present as symptoms related to location – chronic cough, back pain, mass effect, and discharging sinus. Sometimes gossypiboma/RSI may be unnoticed for years and are detected incidentally on imaging studies.
| Risk Factors|| |
Failure to adhere to AORN (Association of perioperative registered nurses) guidelines in counting the swabs and surgical items in open surgeries is one of the risk factors, not exclusive for RSI.
Studies have indicated that females are more prone to the adverse event of retained surgical items. RSI is more frequent with emergency surgeries due to sudden deviations in the plan of surgery or improper swab count. Higher body mass index is also believed to be a contributing factor due to increased technical difficulty, increased stress to the surgeon, and increased surgically exposed area. A recent meta-analysis published in 2014 challenged the contribution of the abovementioned factors to increased incidence by showing no significant statistical difference in incidence with changes in nursing staff, emergency surgery, body mass index, and operation “after hours.”
| Imaging Features|| |
Imaging features depend on the time since surgery, the presence of secondary infection, communication of the gossypiboma with hollow viscus or external skin wound, and the modality of the radiological investigation. A high index of suspicion is needed for diagnosing RSI. Image-guided biopsy or surgical or endoscopic retrieval are confirmatory for retained surgical gauze.
Radiographs are the most commonly used modality to detect gossypibomas., Abdominal radiographs are commonly done postoperatively in patients with pain and abdominal symptoms. Common appearance of retained surgical gauge includes fine linear radio-opacity and associated mottled air or mass effect or density over adjacent soft tissues [Figure 2], [Figure 3], [Figure 4]. The radiopaque marker thread may not always be present as it may slip off or get distorted, twisted, or disintegrated over time. The diagnosis is, however, difficult using only plain radiographs.,, Radiographs can also detect complications such as concurrent intestinal obstruction or perforation. In the presence of a fistula, a contrast study may reveal the location and extent. Other metallic surgical items can be easily identified by their density and shape.
|Figure 2 A-E: Gossypiboma of the abdomen. (A) CT topogram showing a linear hyperdense structure in right hypochondrium. (B-D) coronal, sagittal and axial images of contrast-enhanced CT showing large subphrenic fluid collection with mottled air (yellow arrow head) and a hyperdense structure giving streak artifacts (white arrows). (E) On Ultrasonography, the fluid collection showing echogenic anterior strip with posterior acoustic shadowing|
Click here to view
|Figure 3 (A and B): A case of carcinoma ovary, post-operative abdominal radiograph showing a linear hyperdense structure in the lower abdomen (A). The axial sections of the CT showing poorly defined fluid collection between bowel loops and a hyperdense structure within (B)|
Click here to view
|Figure 4 (A and B): (A) Post colectomy abdomen radiograph showing few linear structures in the lower abdomen (white arrows). Ring shadow of the ileostomy bag is shown by the yellow arrow. (B) Targeted Ultrasonography showing an ill-defined mass with non-dependant air foci (arrowheads). Continued in figure 5|
Click here to view
Computed tomography (CT) is a sensitive method for detecting gossypibomas/RSI and is the next imaging modality of choice if radiographs are negative. The typical imaging appearance in CT is a spongiform pattern with a radiodense linear structure [[Figure 2], [Figure 3], [Figure 5] and [Figure 6]]. Peripheral rind of calcification around a reticular mass, as a characteristic feature of gossypiboma, was described in a report by Lu et al. They found that the “calcified reticulate rind” sign was helpful in identifying the retained gauze piece in chronic cases where the gas bubbles within are gradually absorbed. Another characteristic appearance described is of an inhomogeneous, low-density mass with a thin high-density capsule showing marked enhancement in postcontrast studies.
|Figure 5 (A-D): (Continued from Figure 5). (A-C)The axial and coronal CT scan images showing areas of mottled air surrounded by thick walls. The HU values of internal contents are ranging from -281 to 2048 suggestive of a combination of gauze material, fluid, and air. (D) Contrast-enhanced MRI showing central region of hypointensity with smooth enhancing walls|
Click here to view
|Figure 6 (A-D): Pelvic gossypiboma. CT topogram showing a linear radiodense structure. The axial CT scan images showing the classical appearance of spongiform pattern with thick walls (C). The hyperdense structure causing minimal streak artifacts is seen within the 'wall' of the gossypiboma (B- yellow arrow). The corresponding ultrasonography image is showing an ill-defined cystic structure with an echogenic anterior strip and post acoustic shadowing (D)|
Click here to view
Intrathoracic retained surgical swabs demonstrate well-defined smoothly contoured mass showing peripheral enhancement. As with intra-abdominal gossypiboma, central areas can be heterogeneous due to gas, calcification, and radiopaque markers. Resorption of gas over time is usually seen with gossypibomas in the pleural cavity. Eventually, these appear as pleural-based masses showing atypical calcifications, with the thick, irregular inflammatory walls mimicking chronic infections, granulomatous processes, or neoplasms.,
Small metallic or metal containing RSI can be readily identified and are demonstrated better with maximum intensity projections (MIP) [Figure 7] and [Figure 8].
|Figure 7 (A-D): A case of retained surgical swab in the neck. (A and B) axial CT scan images showing heterogeneous area with air densities and a linear high density representing the radiodense marker of gauze. (Figure A) The HU value of the radiodense material is 1844 and (Figure B) the HU value of the mottled dark area is -163; higher than expected HU value of air (-1000) due to partial volume effect. (C and D) Shaded surface display showing whorled coil of the radiodense marker|
Click here to view
|Figure 8 (A-D): Intrathoracic retained surgical gauze. (A) Frontal chest radiograph showing an ill-defined opacity in left lower zone. After several days, the patient underwent chest CT scan for increased symptoms. (B) Axial images of CT scan showing a linear radiodensity within the area of consolidation. (C and D) MIP images in axial and sagittal reformation. (yellow arrows are showing the hyperdense material of the surgical gauze and the dotted yellow arrows are showing the intercostal drainage tube)|
Click here to view
The sonographic imaging appearances of retained sponge/gauge are classified as three types [Figure 2], [Figure 4], [Figure 6], [Figure 9]A,[Figure 9]B,[Figure 9]C and [Figure 10].
|Figure 9 (A-C): Illustrative diagrams showing the different appearances of gossypiboma on sonography. (A) Poorly defined echogenic area/ echogenic anterior strip with intense posterior acoustic shadowing, (B) A well-circumscribed cystic mass containing internal mottled contents. (C) Non-specific pattern stimulating a complex mass. The posterior acoustic pattern is a consistent finding due to a combination of gauze material, air foci, and calcified regions|
Click here to view
|Figure 10 (A-D): Another case of retained surgical swab in the lower neck, post neck dissection. (A) Radiograph showing irregular linear and specks of densities (white arrow). (B) Sonography image showing cavity showing highly reflective contents with posterior acoustic shadowing. (C and D) Plain and contrast-enhanced axial CT scan images show hyperdense well-defined mass showing intense but heterogeneous contrast enhancement. The central area shows negative HU due to the presence of gas. Note non-visualization of the radiodense marker|
Click here to view
- Poorly defined echogenic area/echogenic anterior strip with intense posterior acoustic shadowing,
- A well-circumscribed cystic mass containing internal mottled contents,
- Nonspecific pattern stimulating a complex mass. The posterior acoustic pattern is a consistent finding due to a combination of gauze material, air foci, and calcified regions.
Magnetic resonance imaging
On magnetic resonance imaging (MRI), a combination of cotton, water, edema due to the inflammatory and fibrous reaction, results in a retained sponge/gauze appearing as a soft-tissue mass with a thick well-defined capsule, having a whorled internal configuration on T2-weighted images. The peripheral calcifications are seen on T1 and T2-weighted images. A complex mass with mixed signal intensity is less commonly seen. Depending on the amount of water content, the signal intensity on T2-weighted images varies from low to high signal intensities. Postcontrast imaging shows peripheral enhancement [Figure 5] and [Figure 11].
|Figure 11 (A-D): Migrated Copper T in pelvis seen on PET-CT. The axial, coronal and sagittal reformatted images of fused PETCT (A-C) and CT images (D-F) showing an FDG uptake around the foreign body which corresponds to inflammatory changes|
Click here to view
With increasing clinical use of PET/CT, few case of imaging appearance has been reported., In reported gossypibomas, low central radiotracer uptake with high peripheral uptake corresponding to active inflammatory reaction near fibrotic capsule has been demonstrated. Low-grade tracer uptake is attributed to inflammatory reaction around the foreign body [Figure 11].
A bone scan with technetium-99m-methylene diphosphonate may show extraosseous accumulation of tracer in sites corresponding to the inflammatory granuloma of RSI.
A retained sponge on mammography appears as high-density mass with varying appearance. The characteristic twisted radiodense marker clinches the diagnosis. Skin thickening and retraction of skin and nipple may be seen as sequelae to non healing wound and chronic fibrosis [Figure 12] and [Figure 13].
|Figure 12 (A-C): A case of retained surgical gauze in breast post surgery. The mammography image showing a high density mass (A). The magnified view shows a few lucent specks (B within the white circle). follow up CT scan showing a mass with low central low HU area in the left breast (C). Continued in Figure 13|
Click here to view
|Figure 13 (A-E): (continued from previous Figure 12). MR images of the breast mass are showing isointensity on T1W images, hyperintensity on T2W and T2 fat sat images (A-C respectively). Post contrast fast T1 image showing smooth peripheral enhancement which is merging with the adjacent post-operative changes (D). Subsequent re-exploration revealed retained gauze (E)|
Click here to view
| Differential Diagnosis|| |
Presented in [Table 1].
Newer retained surgical items
Recent advances in biomedical engineering and robotics have considerably improved the techniques of surgery such as minimally invasive procedures, laparoscopic, endoscopic surgeries, and current robotic surgeries. With these, the incidence of retained small and complex surgical instruments such as broken plastic parts, guidewires, clips, trochar, and other miscellaneous items has increased. Contributing factors for these errors amount to a narrow window of surgical access, improper size of instrument used, and difficulty in handling small and complex instruments.
Most of the newer RSI are not limited to only sponges and towels but also radiolucent items plastic and silicone which pose a challenge in identifying on conventional imaging. CT and USG may help in these situations if there is a strong suspicion.
| Innovations for Reducing the Incidences|| |
New technologies such as “Electronic Computer Assisted Sponge Counting System” and Radio-Frequency identification sponges (RFID) aim to reduce the incidence with the reasoning that technological error is smaller than human error. A randomized control trial by Greenberg et al. in 2008 concluded that the use of automated counting using barcoded surgical sponges improved the detection of miscounted and misplaced sponges. The effectiveness of radiofrequency detection systems has also been demonstrated in some studies.,
The barcode-assisted system includes individual surgical sponges bearing two-dimensional barcodes which are read by a scanner before and after surgery. The count is tracked by a computer and displayed on a screen.
The RFID works on the same principle, however, here the scanning of the sponge does not require optical scanning. Instead, the radiofrequency scanner can detect the RFID bearing sponges, even if it is in the patient's body. The RFID machine consists of a front panel scanner, digital display, out-sponge bucket with a radiofrequency sensor, and a wand to scan the patient's body. Before surgery, all the sponges are counted on a front panel of the machine, and after that, the final count is made by counting the unused sponges and sponges thrown into the radiofrequency sensor bucket. Any sponge left inside the patient body is finally scanned with the wand.
An analysis by Regenbogen et al. in 2008 comparing the six strategies of prevention of RSI including traditional intraoperative radiography and novel technologies showed that the newer technologies, especially the barcode system, was more cost effective than intraoperative radiography in making retained sponges as a “Never-Event.”
Use of intraoperative imaging such as intraoperative radiography, fluoroscopy, and ultrasonography also helps in identifying missing instruments. Intraoperative ultrasound is cost effective in localizing and helps in retrieving lost surgical items. However, not many studies have elaborated the effectiveness of ultrasound as compared to other modalities.
| Conclusion|| |
Due to the varied appearances of RSI, it is difficult to diagnose them on radiographs. Clinical information, coupled with a high index of suspicion, is mandatory for diagnosis. In dubious cases, confirmation with a second imaging modality can be helpful. Radiologists play a key role in diagnosing retained surgical materials. A reasonable knowledge of this entity with its common presentations should be possessed by all radiologists. In fact, RSI should be considered in the differential diagnoses of a mass or nonspecific imaging findings in a postoperative patient.
We would like to thank Dr. Sneha Zanje for help and support in drafting this article.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Patil KK, Patil SK, Gorad KP, Panchal AS, Arora SS, Gautam RP. Intraluminal migration of surgical sponge: Gossypiboma. Saudi J Gastroenterol 2010;16:221-2.
] [Full text]
Gibbs VC. Retained surgical items and minimally invasive surgery. World J Surg 2011;35:1532-9.
World Health Organization. The ICD-10 classification of mental and behavioural disorders: Diagnostic criteria for research. ICD-10 Classif Ment Behav Disord Diagnostic criteria Res; 1993.
Hyslop JW, Maull KI. Natural history of the retained surgical sponge. South Med J 1982;75:657-60.
Goldberg JL, Feldman DL. Implementing AORN recommended practices for prevention of retained Surgical Items. AORN J 2012;95:205-19.
Lata I, Sahu S, Kapoor D. Gossypiboma, a rare cause of acute abdomen: A case report and review of literature. Int J Crit Illn Inj Sci 2011;1:157-60.
] [Full text]
Anderson JM, Rodriguez A, Chang DT. Foreign body reaction to biomaterials. Semin Immunol 2008;20:86-100.
Ranjan R, Nityasha N, Anubhav P, Arya K, Mishra N. Transvisceral migration of gossypiboma presenting as gastric outlet obstruction managed endoscopically. Int Surg J 2016;3:1663-5.
Zantvoord Y, van der Weiden RMF, van Hooff MHA. Transmural migration of retained surgical sponges: A systematic review. Obstet Gynecol Surv 2008;63:465-71.
Chopra S, Suri V, Sikka P, Aggarwal N. A Case Series on Gossypiboma-Varied Clinical Presentations and Their Management. J Clin Diagn Res 2015;9:QR01-3.
Dakubo J, Clegg-Lamptey JN, Hodasi WM, Obaka HE, Toboh H, Asempa W. An intra-abdominal gossypiboma. Ghana Med J 2009;43:43.
Sozutek A, Colak T, Reyhan E, Turkmenoglu O, Akpinar E. Intra-abdominal Gossypiboma Revisited: Various Clinical Presentations and Treatments of this Potential Complication. Indian J Surg 2015;77:1295-300.
Zbara P, Agrawal A, Saeed IT, Utidjian MR. Gossypiboma revisited: A case report and review of the literature. J R Coll Surg Edinb 1998;43:417-8.
Kaiser CW, Friedman S, Spurling KP, Slowick T, Kaiser HA. The retained surgical sponge. Ann Surg 1996;224:79-84.
Botet del Castillo FX, López S, Reyes G, Salvador R, Llaurado JM, Penalva F, et al
. Diagnosis of retained abdominal gauze swabs. Br J Surg 1995;82:227-8.
Gawande AA, Studdert DM, Orav EJ, Brennan TA, Zinner MJ. Risk factors for retained instruments and sponges after surgery. N
Engl J Med 2003;348:229-35.
Moffatt-Bruce SD, Cook CH, Steinberg SM, Stawicki SP. Risk factors for retained surgical items: A meta-analysis and proposed risk stratification system. J Surg Res 2014;190:429-36.
Wan YL, Ko SF, Ng KK, Cheung YC, Lui KW, Wong HF. Role of CT-guided core needle biopsy in the diagnosis of a gossypiboma: Case report. Abdom Imaging 2004;29:713-5.
Manzella A, Borba Filho P, Albuquerque E, Farias F, Kaercher J. Imaging of gossypibomas: Pictorial review. Am J Roentgenol 2009;193.
Chagas Neto FA das, Agnollitto PM, Mauad FM, Barreto AR, Muglia VF, Junior JE. Avaliação por imagem dos gossipibomas abdominais. Radiol Bras 2012;45:53-8.
O'Connor AR, Coakley FV, Meng MV, Eberhardt SC. Imaging of Retained Surgical Sponges in the Abdomen and Pelvis. Am J Roentgenol 2003;180:481-9.
Abdul-Karim FW, Benevenia J, Pathria MN, Makley JT. Case report 736: Retained surgical sponge (gossypiboma) with a foreign body reaction and remote and organizing hematoma. Skeletal Radiol 1992;21:466-9.
Topal U, Gebitekin C, Tuncel E. Intrathoracic gossypiboma. Am J Roentgenol 2001;177:1485-6.
Kokubo T, Itai Y, Ohtomo K, Yoshikawa K, Lio M, Atomi Y. Retained surgical sponges: CT and US appearance. Radiology 1987;165:415-8.
Kim KS. Changes in computed tomography findings according to the chronicity of maxillary sinus gossypiboma. J Craniofac Surg 2014;25:e330-3.
Sistla Chandra S, Ramesh A, Karthikeyan SV, Ram D, Ali SM, Subramaniam RV, et al
. Gossypiboma presenting as coloduodenal fistula-report of a rare case with review of literature. Int Surg 2014;99:126-31.
Wan W, Le T, Riskin L, Macario A. Improving safety in the operating room: A systematic literature review of retained surgical sponges. Curr Opin Anaesthesiol 2009;22:207-14.
Lu YY, Cheung YC, Ko SF, Ng SH. Calcified reticulate rind sign: A characteristic feature of gossypiboma on computed tomography. World J Gastroenterol 2005;11:4927-9.
Machado DM, Zanetti G, Araujo Neto CA, Nobre LF, Meirelles GS, Silva JL, et al
. Thoracic textilomas: CT findings. J Bras Pneumol 2014;40:535-42.
Sheehan RE, Sheppard MN, Hansell DM. Retained intrathoracic surgical swab: CT appearances. J Thorac Imaging 2000;15:61-4.
Sugano S, Suzuki T, Iinuma M, Mizugami H, Kagesawa M, Ozawa K, et al
. Gossypiboma: Diagnosis with ultrasonography. J Clin Ultrasound 1993;21:289-92.
Kim CK, Park BK, Ha H. Gossypiboma in abdomen and pelvis: MRI findings in four patients. AJR Am J Roentgenol 2007;189:814-7.
Yuh-Feng T, Chin-Chu W, Cheng-Tau S, Min-Tsung T. FDG PET CT features of an intraabdominal gossypiboma. Clin Nucl Med 2005;30:561-3.
Yu JQ, Milestone BN, Parsons RB, Doss M, Hass N. Findings of intramediastinal gossypiboma with F-18 FDG PET in a melanoma patient. Clin Nucl Med 2008;33:344-5.
Thomas BG, Silverman ED. Focal Uptake of Tc-99m MDP in a Gossypiboma. Clin Nucl Med 2008;33:290-1.
Yeniceri O, Balci P, Cullu N, Deveer M. Gossipyboma in the Breast: A Rare Case Report. J Med Cases 2014;5:140-2.
Greenberg CC, Diaz-Flores R, Lipsitz SR, Regenbogen SC, Mulholland L, Mearn F, et al
. Bar-coding surgical sponges to improve safety: A randomized controlled trial. Ann Surg 2008;247:612-6.
Rupp CC, Kagarise MJ, Nelson SM, Deal AM, Phillips S, Chadwick J, et al
. Effectiveness of a radiofrequency detection system as an adjunct to manual counting protocols for tracking surgical sponges: A prospective trial of 2,285 patients. J Am Coll Surg 2012;215:524-33.
Williams TL, Tung DK, Steelman VM, Chang PK, Szekendi MK. Retained surgical sponges: Findings from incident reports and a cost-benefit analysis of radiofrequency technology. J Am Coll Surg 2014;219:354-64.
Regenbogen SE, Greenberg CC, Resch SC, Kollengode A, Cima RR, Zinner MJ, et al
. Prevention of retained surgical sponges: A decision-analytic model predicting relative cost-effectiveness. Surgery 2009;145:527-35.
Department of Radio-diagnosis, Tata Memorial Hospital, Mumbai, Maharashtra
Source of Support: None, Conflict of Interest: None
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8], [Figure 9], [Figure 10], [Figure 11], [Figure 12], [Figure 13]